Oxidation of Flavonoids which Promote DNA Degradation Induced by Bleomycin-Fe Complex
نویسندگان
چکیده
منابع مشابه
Breakage of DNA-membrane complex by bleomycin.
The " DNA-membrane complex" (1200S-400S) obtained by lysis of rat ascites hepatoma cells in a low concentration of sodium dodecyl sarcosinate-sodium deoxycholate sedimented through neutral sucrose gradient more rapidly than the free DNA" (200S) which was released by a high concentration of sodium dodecyl sulfate. The former was decomposed by Pronase in the gradient but the latter was not affect...
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The coordination chemistry of the iron(II) complex of the antitumor drug bleomycin has been extensively investigated with a number of spectroscopic and chemical techniques. However, the actual structure of this complex is not established. In this report, we present NMR studies of the paramagnetic Fe(II)BLM and use one- and two-dimensional methods to assign the paramagnetically shifted features ...
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The effect of bleomycin on DNA strand breakage and its repair in cultured mouse fibroblast strain L-P3 (a substrain of L929) was studied. Treatment of L-P3 cells with bleomycin caused single-strand breaks of DNA as revealed by alkaline sucrose gradient centrifugation. The singlestrand breaks of DNA were rejoined within 3 hr when the treated cells were incubated at 37°in a medium free of the an...
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We investigated the amplification of bleomycin-induced DNA cleavage by synthetic pyrrole triamide (PyPyPy) using 32P-labeled DNA fragments obtained from human genes. Peplomycin, a kind of bleomycins, plus Fe(II) caused DNA cleavage at the 5'-GC-3' and 5'-GT-3' sequences (damaged bases are underlined). The addition of PyPyPy enhanced the cleavage at cytosine and thymine residues 3' to consecutiv...
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An anaerobic, quercetin-degrading bacterium was isolated from human feces and identified as Clostridium orbiscindens by comparative 16S rRNA gene sequence analysis. The organism was tested for its ability to transform several flavonoids. The isolated C. orbiscindens strain converted quercetin and taxifolin to 3,4-dihydroxyphenylacetic acid; luteolin and eriodictyol to 3-(3,4-dihydroxyphenyl)pro...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2003
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.26.1108